PL Quality Assessment Protocol（蝦苗質(zhì)量評(píng)估）

冰山

哈哈，首次使用電腦登錄水產(chǎn)頻道發(fā)個(gè)帖子紀(jì)念！ （老外的東西，本人英語不好，大家自己翻譯吧！）

PL Quality Assessment Protocol

n
Gross examination: (in white bowl)

n
1.   Size for PL12 P. vannamei
(>8mm, <300/g
about 0.003g)

n
2.   Size variation (big and small)

n
3.   Benthic behaviour

n
4.   Swimming activity

n
5.   Colour (red/black/brown)

n
Microscopic exam: (sample of 20-30 PL – scoring system)

n
1.   HP (Full/dark, lipid vaculoles 10, if not 5 or 0)

n
2.   Gut (Full, vacuoles, peristalsis 10, 5, 0)

n
3.   Fouling (None 10, medium 5, heavy 0)

n
4.   Deformity/necrosis (None 10, medium 5, heavy 0)

n
5.   MGR in 6th Segment (>75% 10, 50-75% 5, <50% 0)

n
6.   MBV by Malachite in HP (None 10, few 5, lots 0)

n
Scoring: Max 60, pass 50, if < 50 or more than 3 zeros: fail.

冰山

&nbsp;Salinity stress test (300 PL at PL 10-12)
Either: transfer to freshwater for 30 minutes and then back to seawater for 30  minutes >75% OK
Or: 1:1 mix with freshwater and after 2 hours >90% OK

Vibrio bacteria examination
100 PL from tank, sterilize in alcohol, washed, ground up
Streaked by 1 sterile wire loop over TCBS and TSA plates
Incubate at 30-35oC for 18-24 hours
Green colonies <30/plate OK (preferably 0)
Yellow colonies <80/plate OK
No Luminescence on TCBS/TSA OK

PCR testing (for WSSV)
150 PL (preferably weakest shrimp)
Preserve in 70-95% alcohol or ship alive
Use 2-step, nested PCR test
If positive result – reject batch.

冰山

PL Nutrition
Make sure PL that are bought have been well fed, and have received plenty of artemia, HUFAs (esp. EPA, DHA and AA), Lecithin (1%), Cholesterol (1%) Choline chloride (0.1%), Vitamin C (1-3% AAP), Vitamin E (0.01%), Astaxanthin (0.01%) and probiotics (not antibiotics)
Such additions improve stress - resistance and grow-out production

thiefchen

這個(gè)應(yīng)該是 mathew briggs 的課件吧

浪跡天涯

我覺得蝦苗的項(xiàng)目也就是這一些部分，其他的就是看你做到那一個(gè)深度！

冰山

回復(fù) 5# 浪跡天涯


    呵呵，這些標(biāo)準(zhǔn)看似很簡(jiǎn)單，要都做到這些標(biāo)準(zhǔn)就不簡(jiǎn)單了！

大蝦熊

回復(fù) 2# 冰山

很不錯(cuò)，至少有個(gè)標(biāo)準(zhǔn)程序了，我們中國(guó)人喜歡憑經(jīng)驗(yàn)和感覺，西方人喜歡標(biāo)準(zhǔn)化，數(shù)量化，我傾向于后者，如果普遍實(shí)施這種標(biāo)準(zhǔn)，農(nóng)民上當(dāng)?shù)目赡苄跃痛蟠蠼档土恕?/td>

冰山

回復(fù) 7# 大蝦熊


    呵呵，太依賴感覺和經(jīng)驗(yàn)容易掉經(jīng)驗(yàn)的陷阱，被表現(xiàn)迷惑！

浪跡天涯

回復(fù) 7# 大蝦熊


    老師：

         我同意你的看法， 這是蝦苗一般對(duì)健康及成長(zhǎng)監(jiān)控。如果能夠把他普及在各個(gè)大蝦苗場(chǎng)，我們的農(nóng)民就有福了。

原來魚旺

大哥！！有無中文譯音啊！！

冰山

回復(fù) 10# 原來魚旺


    這個(gè)你需要找谷歌幫忙下，我也是找谷哥的。

等風(fēng)

后悔英語沒學(xué)好啊。。。。

我愛養(yǎng)大蝦蝦

這些好像是泰國(guó)正大的操作吧？中國(guó)有沒有這樣的操作呢？