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上帝的愛

A microsporidian parasite, Enterocytozoon hepatopenaei (abbreviated as EHP), is an emerging pathogen for penaeid shrimp. EHP has been found in several shrimp farming countries in Asia including Vietnam, Thailand, Malaysia, Indonesia and China, and is reported to be associated with growth retardation in farmed shrimp. We examined the histological features from infected shrimp collected from Vietnam and Brunei, these include the presence of basophilic inclusions in the hepatopancreas tubule epithelial cells, in which EHP is found at various developmental stages, ranging from plasmodia to mature spores. By a PCR targeting the 18S rRNA gene, a 1.1聽kb 18S rRNA gene fragment of EHP was amplified, and this sequence showed a 100% identity to EHP found in Thailand and China. This fragment was cloned and labeled with digoxigenin-11-dUTP, and in situ hybridized to tissue sections of infected Penaeus vannamei (from Vietnam) and P. stylirostris (Brunei). The results of in situ hybridization were specific, the probe only reacted to the EHP within the cytoplasmic inclusions, not to a Pleistophora -like microsporidium that is associated with cotton shrimp disease. Subsequently, we developed a PCR assay from this 18S rRNA gene region, this PCR is shown to be specific to EHP, did not react to 2 other parasitic pathogens, an amoeba and the cotton shrimp disease microsporidium, nor to genomic DNA of various crustaceans including polychaetes, squids, crabs and krill. EHP was detected, through PCR, in hepatopancreatic tissue, feces and water sampled from infected shrimp tanks, and in some samples of Artemia biomass.

上帝的愛

一個(gè)個(gè)拆開我還可以讀出來，有關(guān)孢子蟲的，大家?guī)徒M一下，目前正在研究這個(gè)問題

狼牙166375

一種微孢子寄生蟲-對蝦腸孢子蟲（縮寫為EHP），是一種針對對蝦的新興病原體，目前已在亞洲包括越南、泰國、馬來西亞、印度尼西亞和中國的幾個(gè)蝦養(yǎng)殖國家發(fā)現(xiàn)，報(bào)道稱該種病原能導(dǎo)致蝦生長遲緩。我們檢查了收集自越南和文萊感染蝦體的組織特征，這其中就包含了嗜堿性夾雜物的肝曲小管上皮細(xì)胞，在這里發(fā)現(xiàn)了EHP從瘧原蟲到成熟孢子的多個(gè)發(fā)育階段，通過PCR特異性擴(kuò)增該EHP的18SrRNA基因，一條長為1.1kbp的EHP18SrRNA基因片段被擴(kuò)增出來，且該片段的基因序列與在泰國和中國發(fā)現(xiàn)的EHP序列顯示出100%的同源性。該基因片段被克隆后被標(biāo)上地高辛-11-dUTP標(biāo)記，然后原位雜交到南美白對蝦（來自越南）和南美白細(xì)腳對蝦（文萊）的感染組織部分，原位雜交的結(jié)果是特異性的，探針只在EHP的細(xì)胞質(zhì)內(nèi)含物中發(fā)生了反應(yīng)，而不是一種與棉花蝦疾病相關(guān)的具褶孢蟲屬的孢子蟲。隨后，我們又針對這個(gè)EHP的18SrRNA基因區(qū)域進(jìn)行了PCR檢測，其結(jié)果顯示對EHP是特異性的,并不與其他兩種孢子蟲病原發(fā)生反應(yīng)，阿米巴和棉花蝦疾病微孢子蟲，也不是其他甲殼類包括多毛類、魷魚、螃蟹和磷蝦的基因組DNA，通過PCR我們在感染蝦塘的蝦肝臟組織、糞便、水樣和一些鹵蟲的樣品中發(fā)現(xiàn)了EHP。

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狼牙166375 發(fā)表于 2015-12-14 10:46
一種微孢子寄生蟲-對蝦腸孢子蟲（縮寫為EHP），是一種針對對蝦的新興病原體，目前已在亞洲包括越南、泰國、 ...

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上帝的愛

狼牙166375 發(fā)表于 2015-12-14 10:46
一種微孢子寄生蟲-對蝦腸孢子蟲（縮寫為EHP），是一種針對對蝦的新興病原體，目前已在亞洲包括越南、泰國、 ...

謝謝狼牙老板，這個(gè)月開始系統(tǒng)研究和處理對蝦親蝦防控腸孢子蟲